#0310-5227) 96-well cell wash head (Molecular Devices Cat. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. accurately over a wide dynamic range. Click on the crosshair and move it so that it is centered over the well image. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. A single-mode plate reader is a good starting point. Detergent Compatible Bradford assay. iD5. Infinite 200 Pro Microplate Reader. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. EMax Plus Microplate Reader, Molecular Devices,96 Well Plate, LLC (CAT#: STEM-LE-0510-LC) Cytation™ 1 Cell Imaging Multi-Mode Reader, Wavelength Range: 200 to 700 nm, BioTek Instruments (CAT#: STEM-MR-0003-LKN) Perkin Elmer EnVision 2105-0020 Multilabel Microplate Reader, Perkin Elmer (CAT#: STEM-S-0723-LGZ)The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. 50 µL of. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Microplate Reader (Molecular Devices cat. in space. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. ValitaTiter Assays Setup Guide 2601A October 2023. For more great deals on Microplate readers by Molecular Devices, please browse our Molecular Devices Microplate readers page. automation for any lab. With various on-board features and capabilities,. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Reactions catalyzed by firefly and *Renilla luciferases. 012 fmol 0. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. No filter change with dual monochromator optics. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. style. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. Technical specifications. . By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. 6:30 . You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. 1. adjust the reader. SpectraDrop High-Throughput Screening (HTS) Kit. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. It measures the optical density (OD) of samples in both 96- and 384-well. 98 VMax R2 = 0. microplate . SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. A multitude of modalities. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. In addition, the iD5 reader measures TRF and FP and. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. 7°C and the bottom to 36. Abstract and Figures. Type: Microplate Reader. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Transfer of an assay to 96-well microplate technology increases throughput significantly. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. 7°C (gradient heating design). 54: 1. Well scanning With well scanning, multiple points within each well can be read, providing a high. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. Reading a Microplate . Please refer to the Obsolete Microplate Reader Models for more information. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Contents of wells were pipetted up and down several times to mix. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Reading Capabilities: cuvette or microplate. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. Description. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. This reader also makes use of a unique temperature-independent methodology for path length correction. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. 3. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. white 96 well plate and 75. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. The eight-channel system delivers both high precision and speed when reading 96-well microplates for endpoint measurements, kinetic assays, and spectral scans. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Next generation SpectraMax® absorbance microplate readers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Molecular Devices' microplate readers. Both readers were heated to 37ºC prior to setting up the assay. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Plate wash time (300 µl). 1. (FP) configured microplate readers. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Read the microplate Step 1: Place the microplate in the reader. Cross-talk < 0. An industry-renowned instrument for monitoring GPCRs and ion channels. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. flexibility of monochromator-based systems. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. #N207) LAL Reagent Water (Lonza cat. 2 W x 44. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. 2 OD at 200 nm; this decreased to 0. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. 5 mm and 15. Paradigm: Delta mP. Open the catalog to page 5. 5. Average StDev (mP) 1. It includes both shaking and incubation functions, and is compatible with 96- well microplates. g. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. When reading at wavelengths. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Incubate the plate at 37 °C for approximately one hour. Learn more about how absorbance is measured, and some key applications that utilize. These robust readers have been placed in labs from. This can increase throughput and efficiency, thus saving time and space in the lab. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Table 1: Categories of heating element design. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. 0. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. 96-well microplate at 200 µL/well. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. In . I contacted Molecular Devices and they quoted me almost $6k to come out and replace. ***Figure 1. You can add your own protocols. for ValitaTiter Plus standards read on Molecular Devices readers. Endpoint, kinetic, spectrum. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. absorbance, fluorescence, and luminescence with the added functionality of. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. 1 OD at 230 nm. #3676). 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. For more sample throughput, the system canRating: 3. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. Standard SBS 96 and 384 well plates (128. ZERO BIAS. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 69: 85. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. While "plate reader" usually refers to the devices described. g. personalized as your research. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. With various on-board features and. Pick the best speed, sensitivity, and price for your needs. Use standard plate unless you see your type listed. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. 6. i3x. Key applications: ELISA, nucleic acid, endotoxin testing. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. D. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. No additional manuals are available. Plates can put in the drawer both in landscape and portrait positions. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 0200-6262. Insert the filled microplate into the drawer, matching well A1 with position A1. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Brand: MOLECULAR DEVICES. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. Published Dec 16, 2020 | Updated Dec 16, 2020. microplates or in a cuvette at a selected. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. #T-0254) Lysozyme (Sigma cat. #G7294). Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. MycoAlert Reagent was added to each well and incubated at room temperature for 5. Keywords: fluorescence polarization, trf, fret, igg. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 3% in white 96- and. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Whatever your needs, you’ll find a microplate reader that can meet them. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. 3 OD at 200 nm. Wide range of applications The VersaMax reader covers. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). A microplate reader as . microplate readers. A. 300 µL of Detergent Compatible Bradford reagent was added to each well. In addition, the iD5 reader measures TRF and FP and. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Both readers were heated to 37ºC prior to setting up . The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. The Absorbance 96 is a uniquely designed microplate reader for assays in 96-well format. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. When the user applies a set temperature of 37°C, the top is heated to 37. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. 1536-well: 0. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. 100 μL/well Steady-Luc working solution was added to the wells. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. M5. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Making it possible to perform kinetic analysis of solid. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. The VersaMax and SpectraMax 190 read 96-well microplates. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesa solid, white, flat-bottom 96-well plate. 01. Molecular Devices VersaMax Microplate Reader Specifications. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. (Molecular Devices cat. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. For more sample throughput, both readers can be easily integrated into full roboticVisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath: Datapaq Express (Vial Rack Bottom Label 2D Reader). The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Protein quantitation Cross-talk < 0. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. System setup and calibration are controlled from within SoftMax Pro Software. Minneapolis, MN, USA), according to the manufacturer's protocol. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Bioz Stars score: 86/100, based on 1 PubMed citations. 0 500 1000 1500 2000 2500 3000 3500 time (sec. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Products : SpectraMax M2/M2e reader, SpectraMax. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. 96, 384, 24, 12, 6 well types. explore more. Reconstitution of reagents. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. 0. If the best wavelengths are unknown, the Gemini EM reader will find. Contents of wells were pipetted up and down several times to mix. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. Growth medium was then replaced with fatty acid uptake solution. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Fluorescence Microplate Reader Comparison Chart. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Multi-Mode Microplate Reader for . microplate reader (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc manufactures this product . 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Molecular Devices i3x. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. M3-M5e_manual. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Specifications for the SpectraMax L microplate reader. Infinite 200 Pro Microplate Reader. San Jose, California, June 5, 2018. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Less background noise gives a better limit of detection and so a relative increase in usable signal. 1 pM. 54: 1. 5°C at 37°C. Transfer of an assay to 96-well microplate technology increases throughput significantly. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. Plate Formats: 6 to 384-well microplates. It also allows for the elimination of standard curves. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Read one sample or up to 384 in a. 384 well polystyrene microplates have plate dimensions of 127. 984. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 1 mL/well of media. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. in a 96-well microplate. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. The FilterMax F3 can read 96-well and 384-well plates. Microplates . #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. July 17, 2023. Please refer to the Obsolete Microplate Reader Models for more information. LVF Monochromator™ : Excitation and Emission. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. Use black microplates. The SpectraMax ABS. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Documentation:1. g. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The device was able to achieve 3σ limits of detection of ca. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. When reading at wavelengths. 125 pM * Specifications listed are for europium 96-well: 0. The OptiMax by Molecular Devices is a tunable microplate reader. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. 0 96 Well Plate. The CellTiter-Glo assay from Promega uses luciferase. 2 OD at 200 nm; this decreased to 0. 96-well-plate-scan-time-2-colors-1-site-well. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 384-well: 0. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. 8 Pages. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. Step 2: Launch SoftMax Pro Software. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Wavelength Range 200 to 999 nm. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. We would like to show you a description here but the site won’t allow us. the assay.